Water quality rapid detection of coliform bacteria paper surface water domestic drinking water underground water source wastewater after water treatment
Water quality rapid detection of coliform bacteria paper surface water domestic drinking water underground water source wastewater after water treatment
Water quality rapid detection of coliform bacteria paper surface water domestic drinking water underground water source wastewater after water treatment
Water quality rapid detection of coliform bacteria paper surface water domestic drinking water underground water source wastewater after water treatment
Water quality rapid detection of coliform bacteria paper surface water domestic drinking water underground water source wastewater after water treatment

Water quality rapid detection of coliform bacteria paper surface water domestic drinking water underground water source wastewater after water treatment

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Water quality rapid detection of coliform bacteria paper surface water domestic drinking water underground water source wastewater after water treatment

About the product

Characteristic

Origin

Guangzhou

Item number

LC01EC

Brand

Litesebang●Measuring biology

Scope of application

It is suitable for rapid determination and counting of coliforms in clean water samples such as surface water, groundwater, source water, drinking water and treated wastewater

Test methods and standards

15-tube mpn

Manufacturer

Liqi biology/haopuer

Description

1、适用范围    
      适用于地表水、地下水、水源水、生活饮用水及经处理后废水等清洁水样中大肠菌群的快速测定与计数。
  2、方法原理    
        将乳糖胆盐选择性培养基和TTC显色剂加载在纸片上,经培养后能够在纸片上生长并发酵乳糖产酸的即为大肠菌群阳性,记录每个稀释度大肠菌群阳性纸片数,根据大肠菌群MPN表查出相应的大肠菌群数。  
       执行标准:
       生活饮用水卫生标准(GB 5749);
      生活饮用水标准检验方法(GB/T       5750.12)。
  3、方法特点   
       与国标法中的十五管法相对应,将传统几步的试验简化为一步法,时间由78 h缩短为18h以内,省去了制备培养基、消毒和培养器皿的清洗处理等大量辅助性工作,随时可以打开包装进行抽样检测。 
 4、操作方法   
  4.1 接种:
     用灭菌吸管吸取10mL待测水样原液插入装有大纸片的塑料薄膜袋中,均匀涂布,共做5个重复;再分别取1mL待测水样原液加到小纸片中,共接5个重复;最后取1mL水样原液加到9mL灭菌生理盐水中混匀(即为10倍稀释待测液),用1mL灭菌吸管分别吸取1mL稀释液(即0.1mL水样),加到最后5片小纸片中。    
  4.2 培养:
      将接种好的纸片放于培养箱中,36℃±1℃培养15~18h。 
 5、结果判读    
      观察每片颜色变化,若纸片变黄或在黄色背景上呈现红色斑点为大肠菌群阳性,若纸片保持紫蓝色不变或在紫蓝色背景上呈现红色斑点,但周围没有黄色均为大肠菌群阴性。                                     
6、结果报告    
      根据阳性反应纸片数,查MPN表可得出水样中大肠菌群的MPN值。如水样污染严重含菌量较多,可将水样稀释成10-1和10-2再加到纸片上,即实际水样加量为1mL、0.1mL、0.01mL水样,其实际MPN值乘以10,以此类推。    
  6.1、列举说明    假设检验15张纸片阳性数分别为5-1-0,那么查水质大肠菌群MPN检索表可知,大肠菌群的MPN值为每100mL水样中33,如果接种量为1mL、0.1mL、0.01mL时,MPN值为330。 
7、保存条件   
     本产品需存放在4℃~10℃冰箱中,保质期为一年,铝箔袋打开后,未用完的纸片要放回铝箔袋中封好,放到冰箱中,一个月内用完。在高湿度的环境中可能出现冷凝水,最好在拆封前将整包回温至室温。

Characters

Properties

Origin

Guangzhou

Item number

LC01EC

Brand

Litesebang●Measuring biology

Scope of application

It is suitable for rapid determination and counting of coliforms in clean water samples such as surface water, groundwater, source water, drinking water and treated wastewater

Description

1、适用范围    
      适用于地表水、地下水、水源水、生活饮用水及经处理后废水等清洁水样中大肠菌群的快速测定与计数。
  2、方法原理    
        将乳糖胆盐选择性培养基和TTC显色剂加载在纸片上,经培养后能够在纸片上生长并发酵乳糖产酸的即为大肠菌群阳性,记录每个稀释度大肠菌群阳性纸片数,根据大肠菌群MPN表查出相应的大肠菌群数。  
       执行标准:
       生活饮用水卫生标准(GB 5749);
      生活饮用水标准检验方法(GB/T       5750.12)。
  3、方法特点   
       与国标法中的十五管法相对应,将传统几步的试验简化为一步法,时间由78 h缩短为18h以内,省去了制备培养基、消毒和培养器皿的清洗处理等大量辅助性工作,随时可以打开包装进行抽样检测。 
 4、操作方法   
  4.1 接种:
     用灭菌吸管吸取10mL待测水样原液插入装有大纸片的塑料薄膜袋中,均匀涂布,共做5个重复;再分别取1mL待测水样原液加到小纸片中,共接5个重复;最后取1mL水样原液加到9mL灭菌生理盐水中混匀(即为10倍稀释待测液),用1mL灭菌吸管分别吸取1mL稀释液(即0.1mL水样),加到最后5片小纸片中。    
  4.2 培养:
      将接种好的纸片放于培养箱中,36℃±1℃培养15~18h。 
 5、结果判读    
      观察每片颜色变化,若纸片变黄或在黄色背景上呈现红色斑点为大肠菌群阳性,若纸片保持紫蓝色不变或在紫蓝色背景上呈现红色斑点,但周围没有黄色均为大肠菌群阴性。                                     
6、结果报告    
      根据阳性反应纸片数,查MPN表可得出水样中大肠菌群的MPN值。如水样污染严重含菌量较多,可将水样稀释成10-1和10-2再加到纸片上,即实际水样加量为1mL、0.1mL、0.01mL水样,其实际MPN值乘以10,以此类推。    
  6.1、列举说明    假设检验15张纸片阳性数分别为5-1-0,那么查水质大肠菌群MPN检索表可知,大肠菌群的MPN值为每100mL水样中33,如果接种量为1mL、0.1mL、0.01mL时,MPN值为330。 
7、保存条件   
     本产品需存放在4℃~10℃冰箱中,保质期为一年,铝箔袋打开后,未用完的纸片要放回铝箔袋中封好,放到冰箱中,一个月内用完。在高湿度的环境中可能出现冷凝水,最好在拆封前将整包回温至室温。

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